About
Stem cells are the diverse class of cells including embryonic stem cells (ESC), tissue-specific stem cells (MSC) and induced pluripotent stem cells (iPS). Previous studies have mainly used methods such as light microscopy, fluorescent probes and tissue sections to study the morphology and function of stem cells. However, these methods have limitations and do not allow a full understanding of the diversity of stem cells, the role in disease modification, and the structural and cellular complexity of stem cells in a given organism. Three-dimensional (3D) imaging techniques have been shown to better preserve gene expression, cell polarity, and cell-to-cell contact than traditional two-dimensional (2D) cell culture techniques.
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